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v v pen strep thermo fisher  (Thermo Fisher)


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    Structured Review

    Thermo Fisher v v pen strep thermo fisher
    V V Pen Strep Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v+v+pen+strep+thermo+fisher/2+MERCAPTOETHANOL/pm41598984-36-85-87
    Average 99 stars, based on 1 article reviews
    v v pen strep thermo fisher - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Delineation of the antibody light chain biases and kinetics following primary and secondary influenza virus infections of ferrets.
    Article Snippet: Purity of the rHA proteins was confirmed by SDS-PAGE and Coomassie blue (Thermo Fisher). .. Anti-ferret Igκ and Igλ mAb production Murine B-cell hybridomas, anti-ferret Igκ (4-B10), anti-ferret Igλ (8-H9), and anti-ferret IgG (11-E3), secreting anti-ferret IgL-specific monoclonal antibodies (mAbs), were generated and characterized previously.28 For cell expansion and purification, an aliquot (1 × 106 cells per vial) of each hybridoma population was expanded to 1 × 106 cells/mL in 200 mL of B-cell growth medium (RPMI 1640 medium [Sigma-Aldrich] supplemented with 10% [v/v] fetal bovine serum [FBS; Atlanta Biologicals, Flowery Branch, GA, USA], 7.5 mM HEPES [Thermo Fisher], 1% [v/v] Pen/Strep [Thermo Fisher], 50 μM 2-mercaptoethanol [2-ME; Sigma-Aldrich], 1% [v/v] nonessential amino acids [NEAA; Thermo Fisher], 2% [v/v] amino acids [AA; Thermo Fisher], and 1 mM sodium pyruvate [Thermo Fisher]), then transferred to a low-Ig growth medium (RPMI 1640 supplemented with 2% [v/v] low-Ig FBS [Thermo Fisher], HEPES, Pen/Strep, 2-ME, NEAA, AA, and sodium pyruvate), and allowed to secrete antibodies for approximately 30 d in roller bottles (Corning, Corning, NY, USA) at 37 �C. ..

    Generated:

    Article Title: Delineation of the antibody light chain biases and kinetics following primary and secondary influenza virus infections of ferrets.
    Article Snippet: Purity of the rHA proteins was confirmed by SDS-PAGE and Coomassie blue (Thermo Fisher). .. Anti-ferret Igκ and Igλ mAb production Murine B-cell hybridomas, anti-ferret Igκ (4-B10), anti-ferret Igλ (8-H9), and anti-ferret IgG (11-E3), secreting anti-ferret IgL-specific monoclonal antibodies (mAbs), were generated and characterized previously.28 For cell expansion and purification, an aliquot (1 × 106 cells per vial) of each hybridoma population was expanded to 1 × 106 cells/mL in 200 mL of B-cell growth medium (RPMI 1640 medium [Sigma-Aldrich] supplemented with 10% [v/v] fetal bovine serum [FBS; Atlanta Biologicals, Flowery Branch, GA, USA], 7.5 mM HEPES [Thermo Fisher], 1% [v/v] Pen/Strep [Thermo Fisher], 50 μM 2-mercaptoethanol [2-ME; Sigma-Aldrich], 1% [v/v] nonessential amino acids [NEAA; Thermo Fisher], 2% [v/v] amino acids [AA; Thermo Fisher], and 1 mM sodium pyruvate [Thermo Fisher]), then transferred to a low-Ig growth medium (RPMI 1640 supplemented with 2% [v/v] low-Ig FBS [Thermo Fisher], HEPES, Pen/Strep, 2-ME, NEAA, AA, and sodium pyruvate), and allowed to secrete antibodies for approximately 30 d in roller bottles (Corning, Corning, NY, USA) at 37 �C. ..

    Purification:

    Article Title: Delineation of the antibody light chain biases and kinetics following primary and secondary influenza virus infections of ferrets.
    Article Snippet: Purity of the rHA proteins was confirmed by SDS-PAGE and Coomassie blue (Thermo Fisher). .. Anti-ferret Igκ and Igλ mAb production Murine B-cell hybridomas, anti-ferret Igκ (4-B10), anti-ferret Igλ (8-H9), and anti-ferret IgG (11-E3), secreting anti-ferret IgL-specific monoclonal antibodies (mAbs), were generated and characterized previously.28 For cell expansion and purification, an aliquot (1 × 106 cells per vial) of each hybridoma population was expanded to 1 × 106 cells/mL in 200 mL of B-cell growth medium (RPMI 1640 medium [Sigma-Aldrich] supplemented with 10% [v/v] fetal bovine serum [FBS; Atlanta Biologicals, Flowery Branch, GA, USA], 7.5 mM HEPES [Thermo Fisher], 1% [v/v] Pen/Strep [Thermo Fisher], 50 μM 2-mercaptoethanol [2-ME; Sigma-Aldrich], 1% [v/v] nonessential amino acids [NEAA; Thermo Fisher], 2% [v/v] amino acids [AA; Thermo Fisher], and 1 mM sodium pyruvate [Thermo Fisher]), then transferred to a low-Ig growth medium (RPMI 1640 supplemented with 2% [v/v] low-Ig FBS [Thermo Fisher], HEPES, Pen/Strep, 2-ME, NEAA, AA, and sodium pyruvate), and allowed to secrete antibodies for approximately 30 d in roller bottles (Corning, Corning, NY, USA) at 37 �C. ..

    Article Title: Delineation of the antibody light chain biases and kinetics following primary and secondary influenza virus infections of ferrets
    Article Snippet: .. For cell expansion and purification, an aliquot (1 × 10 6 cells per vial) of each hybridoma population was expanded to 1 × 10 6 cells/mL in 200 mL of B-cell growth medium (RPMI 1640 medium [Sigma-Aldrich] supplemented with 10% [v/v] fetal bovine serum [FBS; Atlanta Biologicals, Flowery Branch, GA, USA], 7.5 mM HEPES [Thermo Fisher], 1% [v/v] Pen/Strep [Thermo Fisher], 50 μM 2-mercaptoethanol [2-ME; Sigma-Aldrich], 1% [v/v] nonessential amino acids [NEAA; Thermo Fisher], 2% [v/v] amino acids [AA; Thermo Fisher], and 1 mM sodium pyruvate [Thermo Fisher]), then transferred to a low-Ig growth medium (RPMI 1640 supplemented with 2% [v/v] low-Ig FBS [Thermo Fisher], HEPES, Pen/Strep, 2-ME, NEAA, AA, and sodium pyruvate), and allowed to secrete antibodies for approximately 30 d in roller bottles (Corning, Corning, NY, USA) at 37 °C. ..



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